Journal article
Effect of Lipid-Based Nanostructure on Protein Encapsulation within the Membrane Bilayer Mimetic Lipidic Cubic Phase Using Transmembrane and Lipo-proteins from the Beta-Barrel Assembly Machinery
L Van 'T Hag, HH Shen, TW Lin, SL Gras, CJ Drummond, CE Conn
Langmuir | AMER CHEMICAL SOC | Published : 2016
Abstract
A fundamental understanding of the effect of amphiphilic protein encapsulation on the nanostructure of the bicontinuous cubic phase is crucial to progressing biomedical and biological applications of these hybrid protein-lipid materials, including as drug delivery vehicles, as biosensors, biofuel cells and for in meso crystallization. The relationship between the lipid nanomaterial and the encapsulated protein, however, remains poorly understood. In this study, we investigated the effect of incorporating the five transmembrane and lipo-proteins which make up the β-barrel assembly machinery from Gram-negative bacteria within a series of bicontinuous cubic phases. The transmembrane β-barrel Ba..
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Grants
Awarded by Commonwealth Scientific and Industrial Research Organisation
Funding Acknowledgements
The authors acknowledge and respect Professor Toyoki Kunitaki for his immense contributions to the field of molecular self-assembly. He has served as a role model and as an inspiration to many researchers across the globe. We thank Drs. Adrian M. Hawley, Stephen T. Mudie, and Nigel M. Kirby for their assistance with SAXS experiments, and we acknowledge use of the SAXS/WAXS beamline at the Australian Synchrotron. We thank Dr. Janet Newman for help with the robotic setup of plates at the C<SUP>3</SUP> Collaborative Crystallization Centre, CSIRO, Parkville, Australia. A/Prof. Sally L. Gras is supported by the ARC Dairy Innovation Hub IH120100005. Dr. Charlotte Conn is the recipient of an ARC DECRA Fellowship DE160101281. Dr. Hsin-Hui Shen is supported by the NMHRC CDF GNT1106798. Leonie van 't Hag is supported by a Melbourne International Research Scholarship and a CSIRO PhD scholarship. Prof. Ian Henderson provided the BamB-E plasmids.